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Objective:To discuss the relationships between the expressions of silence information regulator 6 (SIRT6) and survivin and clinicopathological features of gastric cancer, and to investigate their effects in gastric cancer.Methods:The tumor tissues of 110 gastric cancer patients admitted to the First Affiliated Hospital of Xi′an Jiaotong University from March 2013 to October 2014, as well as 40 adjacent tissues and 20 normal tissues, were selected to detect the expressions of SIRT6 and survivin by immunohistochemistry. The correlations between the expression levels of SIRT6 and survivin and the clinicopathological features and prognosis of gastric cancer patients were analyzed.Results:The positive rates of SIRT6 were 41.8% (46/110), 77.5% (31/40) and 85.0% (17/20) in gastric cancer, adjacent tissues and normal gastric tissues, respectively, and the difference among the three groups was statistically significant ( χ2=23.200, P<0.001). The positive rate of SIRT6 in gastric cancer tissue was lower than that in adjacent and normal tissues ( χ2=14.949, P<0.001; χ2=12.634, P<0.001). The expression of SIRT6 was correlated with tumor differentiation ( χ2=19.654, P<0.001). The positive rates of survivin were 58.2% (64/110), 15.0% (6/40) and 0 (0/20) in gastric cancer, adjacent tissues and normal gastric tissues, respectively, and the difference among the three groups was statistically significant ( χ2=38.449, P<0.001). The positive rate of survivin in gastric cancer tissue was higher than that in adjacent and normal tissues ( χ2=21.976, P<0.001; χ2=22.920, P<0.001). The expression of survivin was correlated with the depth of infiltration ( χ2=20.853, P<0.001). The expression of SIRT6 was correlated with survivin in gastric cancer tissues ( C=0.211, P=0.024). Survival analysis showed that 3-year survival rate was 53.1% in the SIRT6 negative patients, lower than 78.3% in the positive patients, and the difference was statistically significant ( χ2=4.004, P=0.045), while the 3-year survival rates of the survivin positive and negative patients were 53.1% and 78.3%, and the difference was not significant ( χ2=3.717, P=0.054). Cox multivariate regression analysis showed that lymph node metastasis ( RR=6.618, 95% CI: 2.152-20.358, P=0.001) and SIRT6 negative expression ( RR=0.228, 95% CI: 0.081-0.644, P=0.005) were the risk factors for poor prognosis of gastric cancer. Conclusion:SIRT6 is poorly expressed in gastric cancer tissues and is related to the prognosis of gastric cancer, while survivin is highly expressed in gastric cancer tissues. The expression of SIRT6 and survivin is negatively correlated, suggesting that the expression imbalance of SIRT6 and survivin may play an important role in the occurrence and development of gastric cancer.
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ABSTRACT:Objective To investigate the effects of total flavones of oldenlandia diffusa (FOD)on epithelial-mesenchymal transition in hepatocellular cancer cell line MHCC97-H.Methods TGF-β1 induced EMT in routinely cultured liver cancer cell line MHCC97-H;then MHCC97-H cell was divided into 5 groups:normal control group, TGF-β1 group,TGF-β1 + FOD group,TGF-β1 + 5-FU group,and TGF-β1 + FOD + 5-FU group.After 48 h of treatment,the invasion ability of MHCC97-H cell was detected by Transwell;the proteins of E-cadherin and vimentin were determined by Western blot.Results Compared with the normal form of MHCC97-H cell line,the cell had obvious long fusiform after TGF-β1 induction,and the invasion ability enhanced (P = 0.02 ).But after treatment,the invasion ability of MHCC97-H cell decreased in FOD group and 5-FU group compared with that in TGF-β1 group (P = 0.03,P = 0.02 ),and decreased more significantly in FOD + 5-FU group (P = 0.01 ).The expression of E-cadherin at the protein level decreased significantly (P = 0.01 )in TGF-β1 group,which was abolished in FOD group (P =0.03 )and 5-FU group (P = 0.02 ).The expression of vimentin at the protein level increased significantly (P =0.01)in TGF-β1 group,which was abolished in FOD group (P =0.04)and 5-FU group (P =0.03)and more obviously in FOD+5-FU group (P =0.01).Conclusion FOD can reverse the invasion of MHCC97-H cells in EMT induced by TGF-β1 through decreasing the expression of E-cadherin protein and inhibiting the epithelial-mesenchymal transition of MHCC97-H cell.
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Objective To observe the effects of chemo-endocrine therapy on gastric carcinoma (GC). Methods Cancerous tissues from 95 patients with primary GC were examined for the presence of estrogen receptor (ER) and progesterone receptor (PgR). Afterwards, 64 GC patients with recurrent or metastatic conditions were randomly divided into two groups: chemotherapy group and chemo-endocrine therapy group. Results A total of 34.38% ER positive and 16.30% PgR positive rates were found. For ER positive patients, the use of tamoxifen-EAP regimens was more effective than that of EAP alone (P
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Objective To evaluate the efficacy and safety of topotecan(TPT) in the treatment of advanced epithelial ovarian carcinoma. Methods 84 patients with advanced epithelial ovarian carcinoma received TPT(1.25mg/m~2) as a 30-minute infusion daily for 1-5 days,21 days for a cycle.The efficacy was evaluated after 2 cycles of chemotherapy.Response was confirmed 4 weeks later.Results In 84 selected patients,72 were assessable for response and 84 for toxicity.The overall response was 22.2%,including 2 CR and 14 PR.The response rate for untreated and recurrent advanced epithelial ovarian carcinoma was 25.0% and 20.8%,respectively.The main side effects were neutropenia and leukopenia.WHO grade III-IV of them were 26.1% and 26.1%,respectively.The non-hemotological toxicity was mild.Conclusion TPT is effective and well-tolerated in the treatment of advanced epithelial ovarian carcinoma,especially in recurrent patients.